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. 2014 Feb 18;105(4):418–424. doi: 10.1111/cas.12356

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Fig. 1 — RUNX3-induced Wnt signaling activation. (a) Western blotting of the RUNX3 expression in gastric cancer cells. (b) Relative luciferase activity of TOPflash/FOPflash in the respective gastric cancer cells transfected with RUNX3 or R122C mutant RUNX3 vector to the control vector-transfected cells. (c) Luciferase activity of the TOPflash and FOPflash in KatoIII-R3 cells and siRNA-transfected KatoIII-R3 cells relative to the level in the parental KatoIII cells. (d) Relative luciferase activity of TOPflash/FOPflash in KatoIII cells transfected with RUNX3 expression vector. (e) Relative expression levels of the Wnt target genes, SOX4 and Axin2, detected by RT-PCR in the RUNX3 vector-transfected cells (red) or RUNX3 siRNA-transfected cells (blue) to the control level. (f) Relative luciferase activity of TOPflash/FOPflash in AZ521 and MKN45 cells transfected with RUNX3 siRNA to the control level. (b–f) Bar graphs are shown as mean ± SD. *P < 0.05 versus control levels otherwise indicated.