Fig. 1.

Immunohistochemistry (IHC) of glypican-3 (GPC3) in hepatocellular carcinoma. (a) Representative GPC3 staining features observed in matched specimens evaluated using two IHC methods. Method 1 was used in a previous first-in-human study; method 2 was a fully automated IHC assay. Scores are indicated under each photograph. Bar = 50 μm (b) Comparison of total GPC3 staining score by method 1 (0–14) and clinical score by method 2 (0–3). Spearman's correlation was 0.76 (P = 0.00255). (c) Comparison of clinical score and membrane H score. (d) Comparison of clinical score and cytoplasm H score. There was a positive association between the H scores and the GPC3 clinical scores, with R² values of 0.75 and 0.33, respectively.