Table 1.

Scoring system of immunohistochemical (IHC) staining of glypican-3 (GPC3) in hepatocellular tumor specimens using method 1

	Score
Positive cell rate (PR)
No staining	0
<20%	1
20–49%	2
≥50%	3
Staining intensity (SI)†
Cytoplasm (SI-Cp)
No staining	0
Minimal staining	1
Cell membrane (SI-Cm)
Weak staining	2
Moderate staining	3
Strong staining	4
Staining pattern of cell membrane (SP-Cm)‡
No cell membrane pattern	0
Incomplete or partly complete (<20%)	1
Focally complete (20–49%)	2
Rather complete (≥50%)	3
GPC3 staining scoring	IHC total = PR + SI-Cp + SI-Cm + SP-Cm

^†Scoring method 1, used in a previous first-in-human study, involved assessment by three qualified pathologists blinded to clinical outcomes using the scoring criteria shown here. †GPC3 staining categories: minimal, slight staining recognized at low-power objective, ×10; weak, slight staining recognized at low-power objective, ×4; moderate, staining found easily at ×4, but weaker than that of strong staining; strong, staining apparent strong with low-power objective, ×4.

^‡Complete staining, circular (ring-like) staining of tumor cells; incomplete staining, focal staining of rim of cells.