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. 2014 Jan 8;105(2):228–235. doi: 10.1111/cas.12329

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© 2013 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is noncommercial and no modifications or adaptations are made.

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Fig. 2 — MicroRNA-143 (miR-143) expression in scirrhous type gastric cancer (GC) tissue and cell lines. In situ hybridization of miR-143 was carried out in combination with immunofluorescence staining in scirrhous type GC. MicroRNA-143 labeling was revealed by Cy3-conjugated streptavidin (red). (a) α-Smooth muscle actin (α-SMA), (b) vimentin, or (c) CAM5.2 labeling was revealed by FITC-conjugated secondary antibody (green). DNA was counterstained with DAPI (blue). MicroRNA-143 expression was localized to α-SMA-positive and vimentin-positive stromal fibroblasts. (d) MicroRNA-143 expression levels were evaluated in GC cell lines and fibroblasts. Bars and error bars indicate median and standard error, respectively. *P < 0.05. CaF, cancer-associated fibroblasts; NF, normal gastric fibroblasts.