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. 2014 Jan 8;105(2):228–235. doi: 10.1111/cas.12329

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© 2013 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is noncommercial and no modifications or adaptations are made.

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Fig. 3 — Regulation of collagen type III expression by microRNA-143 (miR-143). (a) Collagen type III expression was assessed by immunohistochemical analysis of scirrhous type gastric cancer (GC) tissue. (b) Collagen type III mRNA expression levels were evaluated in GC and fibroblasts. Normal gastric fibroblasts and cancer-associated fibroblasts (NF-38 and CaF-38, respectively) were transfected with negative control miRNA or precursor miR-143 or miR-143 inhibitor, and (c) quantitative RT-PCR, (d) Western blot, and (e) cell staining were carried out for collagen type III expression. Scale bars: 50 μm. (f) Proliferation activity after coculture of HSC-44PE GC cells and CaF-38 with miR-143 inhibitor or negative control. Proliferation activity of HSC-44PE was assessed by percentages of BrdU/CAM5.2-positive cells. (g) Proliferation activity after coculture of HSC-44PE and CaF-38 with COL3A1 siRNA or negative control. Collagen type III expression level was determined by Western blot analysis. Results are mean ± SE of triplicate measurements. *P < 0.05.