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. 2014 Jan 2;105(2):176–185. doi: 10.1111/cas.12331

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© 2013 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 5 — The tumor tissue necrosis and inhibition of angiogenesis in vivo. (a) Representative hematoxylin and eosin-stained tumor tissues obtained from mice in the control (i, iv), 2 mg/kg treated group (ii, v) and 10 mg/kg treated group (iii, vi). (b) Detection of blood vessels via immunohistochemical staining for Von Willebrand factor (VWF) from the control group (i), 2 mg/kg treated group (ii), 10 mg/kg treated group (iii). (c) Quantification of angiogenesis assessed by microvessel density (MVD). Data represent the mean ± standard error (SE), n = 4. (d) The effect of macrophage inhibitory factor (MIC) and anti-MIC1 antibody on human umbilical vein endothelial cells (HUVECs) in vitro; Data represent the mean ± SE, n = 4.