Fig. 2.

Intraperitoneal injection of anti-glucocorticoid induced TNF receptor (GITR) monoclonal antibody (mAb) suppresses the growth of pancreatic cancer tumors. (a) Pan02 cells were inoculated on the right legs in C57BL/6 mice, and 6 days later 100 μg of anti-GITR mAb was intraperitoneally injected into the mice (n = 3). Data are representative of two separate experiments with similar results. (b) The increase of interferon (IFN)-γ-positive cells in response to stimulation of Pan02 cells by an ELISpot assay. Two weeks after the injection of anti-GITR mAb, splenocytes were isolated from treated mice, and co-cultured with Pan02 cells or control lymphocytes (n = 3).