Fig. 4.

The increase of interferon (IFN)-γ-positive cells in response to stimulation of Pan02 cells by the combination therapy. (a) ELISpot assay of IFN-γ-producing cells in response to stimulation of Pan02 cells. Fourteen days after virus injection, splenocytes were isolated from mice, and co-cultured with Pan02 cells or control lymphocytes (n = 3). Data are representative of three separate experiments with similar results. (b) Intracellular cytokine staining of IFN-γ-producing cells in response to stimulation of Pan02 cells. The splenocytes from treated mice were incubated with Pan02 cells and stained by APC-anti-mouse IFN-γ. The activated cell fractions were analyzed by staining with fluorescein isothiocyanate (FITC)-anti-mouse CD4 or CD8 antibody (n = 3).