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. 2014 Apr 3;105(5):553–559. doi: 10.1111/cas.12391

Figure 2.

Figure 2

Receptor activator of factor-κB ligand (RANKL) promotes metastatic colonization of murine osteosarcoma LM8 cells in the bone. (a) Experimental protocol. PBS (Control) or RANKL (1 mg/kg) was injected i.p. at the indicated days after intracardiac (i.c.) transplantation of LM8/luc. Two treatment schedules, RANKL1 and RANKL2, are indicated. Mice were dissected 14 days after i.c. injection of LM8. (b) Representative micro X-ray computed tomography cross-section images of mouse femur 3 days after daily injection of PBS (control) or RANKL (1 mg/kg) (n = 5). *P < 0.05. (c) Representative bioluminescence (BL) in vivo images and quantitative analysis of bone metastasis in hind limbs. RANKL1 (left panel): n = 7 for Control; n = 19 for RANKL1); RANKL2 (right panel): n = 6 for both Control and RANKL2. *P < 0.05. (d) Bone metastasis frequency. RANKL1: n = 7 for Control; n = 11 for RANKL1; RANKL2: n = 4 for Control and RANKL2. *P < 0.05. N.S., not significant.