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Establishment of N-nitrosopyrrolidine (NPYR)-induced transformation in H8 cells. Cells treated with NPYR were seeded in soft agar, and colony numbers were counted 2 weeks after seeding. Untreated H8 cells and SiHa cells were seeded as negative and positive controls, respectively. (a) Representative images taken under a light microscope (40×). (b) Colony numbers in each group counted in five fields (mean ± SD).
