Figure 3.

Increased inhibitors of differentiation-1 (Id-1) expression in N-nitrosopyrrolidine (NPYR)-transformed H8 cells. (a) Total proteins extracted from H8, NPYR-H8 and SiHa cells were subjected to 10% SDS-PAGE gels and assayed by western blot. (b) Id-1 RNA levels in H8, NPYR-H8 and SiHa cells were detected by qRT-PCR. Expression of Id-1 gene, in each sample was normalized against the B2M endogenous control. The normalized values were then calibrated against the H8 cell values that were arbitrarily set as 1.