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High-performance liquid chromatography (HPLC) of the temozolomide (TMZ) released from fibrin glue (FG). (a) HPLC of the calibration curve standards. (b) HPLC of PBS incubated for 24 h with FG sheets containing TMZ (TMZ-FGS). (c) Time-course of the total amount of TMZ released from FG (n = 3). (d) Ratio of TMZ released from FG in the course of 168 h (n = 3). For analysis we used the unpaired two-tailed t-test. *P < 0.05. **P < 0.001. NS, not significant.
