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. 2014 Jul 25;105(8):1032–1039. doi: 10.1111/cas.12447

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 3 — Knockdown of c-MET expression by siRNA in small-cell lung cancer cells. (a) c-MET gene expression was examined using real-time RT-PCR. (b) c-MET and p-MET protein expression was determined using Western blotting. (c) Growth inhibition of stepwise 7-ethyl-10-hydroxycamptothesin (SN-38) or paclitaxel (TXL) concentration in SN-38-resistant (PC-6/SN-38) or TXL-resistant (PC-6/TXL) cells transfected with c-MET siRNA (si MET; ▴) or negative-control (▪) was examined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) assay.