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. 2014 Jul 25;105(8):1032–1039. doi: 10.1111/cas.12447

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 6 — Hepatocyte growth factor (HGF) protein expression in cytotoxic anticancer drug-resistant small-cell lung cancer cells, including PC-6 and NCI-H69 parental cells and those resistant to 7-ethyl-10-hydroxycamptothesin (PC-6/SN-38), paclitaxel (PC-6/TXL and H69/TXL), and cisplatin (PC-6/CDDP). (a) Levels of HGF protein expression in cytotoxic anticancer drug-resistant cells were examined by Western blotting. (b) Secretion of HGF in resistant cells was quantified by quantitative ELISA and compared to that in parental cells. (c) Growth inhibition of SN-38 to PC-6 cultured with or without HGF (50 ng/mL) for 2 weeks was examined with the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt) assay. *P < 0.05; **P < 0.01.