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. 2014 Jul 27;105(8):1063–1070. doi: 10.1111/cas.12458

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 6 — Gambogic acid (GA) inhibited tumor angiogenesis in U266 xenograft mouse model. (a) Immunohistochemistry was performed in tumor sections with antibodies of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and CD31. The result showed a remarkable decrease in expression of HIF-1α, VEGF and CD31 in the treated groups with 2 and 4 mg/kg GA compared with untreated control groups. (b) To identify the tumor angiogenesis, the stained area of CD31 in 10 fields was quantified by using Image Pro Plus. Bars are the mean ± SD (n = 10). The comparisons were made relative to untreated controls, and the different levels of significance was indicated as **P < 0.01. (c) The images were quantified using Image Pro Plus and mean optical densities (of control) of VEGF and HIF-1α were shown. Bars are the mean ± SD (n = 10). The comparisons were made relative to untreated controls, and the different levels of significance was indicated as **P < 0.01.