Fig. 2.

Upregulation of expression of lncRNA-Smad7 by TGF-β. (a) Effect of TGF-β on expression of lncRNA-Smad7 in mouse JygMC(A) and 4T1 breast cancer cells. Cells were treated with 1 ng/mL TGF-β for the indicated periods, and expression of lncRNA-Smad7 relative to that of TATA box binding protein (Tbp) was determined by quantitative RT-PCR (qRT-PCR). (b) Effect of TGF-β and the TGF-β type I receptor kinase inhibitor SB431542 (SB) on lncRNA-Smad7 expression. Cells were treated with 1 ng/mL TGF-β or 10 μM SB431542 for 48 h or left untreated. Relative expression of lncRNA-Smad7 was determined as in (a). Error bars: standard deviations.