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. 2014 Aug 11;105(8):933–942. doi: 10.1111/cas.12459

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 1 — Elevated frequencies of Th17 cells and reduced frequencies of Th1 cells in freshly isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs) from acute myeloid leukemia (AML) patients. (a) PBMCs and BMMCs were isolated from AML patients and healthy donors (HDs) and stimulated for 5 h with phorbol 12-myristate13-acetate (PMA) and ionomycin in the presence of brefeldin A and then stained for CD3, CD8, intracellular interleukin (IL)-17A and interferon (IFN)-γ. Frequencies of Th17 cells and Th1 cells were determined by flow cytometry. Representative dot plots using matching peripheral blood (PB) and bone marrow (BM) samples from AML patients and HDs were shown. (b) Collective results presented for Th17 and Th1 cells within CD4⁺ T population. (c) Total RNA was isolated from CD4⁺ T cells obtained from AML patients and HDs and reverse transcribed into cDNA and subsequently real time polymerase chain reaction (PCR) for IL-17A and IFN-γ. Results were expressed as mean ± SEM.