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. 2014 Aug 11;105(8):933–942. doi: 10.1111/cas.12459

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Fig. 2 — Phenotype of tumor-infiltrating Th17 cells. After stimulation with phorbol 12-myristate13-acetate (PMA) and ionomycin for 5 h, freshly isolated bone marrow mononuclear cells (BMMCs) were subjected to membrane and intracellular staining and analyzed by flow cytometry. Representative data were shown from 21 untreated AML patients. (a) Interleukin (IL)-17A expression in T and B cells. IL-17A expression was analyzed in BMMCs. (b) The expression of HLA-DR, CD25, CCR6, CD45RA, CD45RO, and CD62L in tumor-infiltrating Th17 cells.