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. 2014 May 27;105(6):690–696. doi: 10.1111/cas.12417

Figure 3.

Figure 3

(a) Stathmin1 (STMN1) bound to p27 in the cytoplasm of HuCCT1 cells. Primary STMN1 rabbit and p27 mouse antibodies bound to STMN1 and p27 complexes were combined with secondary proximity ligation assay probes. The interaction events are visible as red dots (nuclear staining with DAPI). (b) siRNA-mediated STMN1 suppression. STMN1 protein levels were measured using western blotting after transfection with STMN1 siRNA. p27 expression in STMN1 siRNA-transfected and untreated HuCCT-1 cells was assessed using western blotting of total and nuclear protein. β-Actin was used as a loading control for total protein and Histone H1 was used as a loading control for nuclear protein. (c) HuCCT-1 cells were transfected with STMN1 siRNA and WST-8 proliferative assays were performed and compared with untransfected cells. (d) Paclitaxel sensitivity was determined using a WST-8 assay. STMN1 siRNA transfection significantly increased paclitaxel sensitivity compared with untransfected cells. Each time point represents the mean ± SD of triplicate determinations in two independent experiments. *P < 0.05.