Figure 1.

Capn4 expression in nasopharyngeal carcinoma (NPC)biopsy tissues and cell lines. (a) mRNA levels of Capn4 in tumor tissues from seven patients with NPC and two normal tissues were determined by RT-PCR. The results were normalized against mRNA levels of β-actin in each sample. N, normal tissues; T, tumor tissues. (b) Western blot analysis on Capn4 expression was performed in NPC tumor tissue samples and normal tissues. Total proteins were extracted from tissues and subjected to immunoblotting probed by antibodies against Capn4. β-actin was probed as control. (c, d) Capn4 mRNA and protein levels were monitored in the NPC cell lines (5-8F, CNE2, and 6-10B) and the immortalized human nasopharyngeal epithelial cell line NP69, respectively. *P < 0.05.