Figure 4.

Matrix metalloproteinase 2 (MMP2) functionally contributes to Capn4-mediated nasopharyngeal carcinoma (NPC) cell migration and invasion. (a) Western blot analysis was performed to monitor protein levels of genes associated with cell migration and invasion in Capn4 siRNA and control siRNA 5-8F cells. (b) mRNA levels of MMP2 were determined by RT-PCR analysis in Capn4 siRNA and control siRNA cells. (c) MMP2 enzyme activity was measured by gelatin zymography in Capn4 siRNA and control siRNA cells. (d) 5-8F and CNE2 cells were transfected with constructs encoding Capn4 or MMP2 siRNA, or full-length MMP2, respectively. Western blot analysis was then performed to monitor knockdown or overexpression of target genes. Data is presented as means ± SD for three independent experiments (*P < 0.05).