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. 2014 May 21;105(7):875–882. doi: 10.1111/cas.12426

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effects of glabridin (GLA) on the epithelial–mesenchymal transition (EMT) markers in MDA-MB-231 and BT-549 cells. (a) Morphological images of MDA-MB-231 cells exposed to 0 or 10 μM GLA for 4 days. (b) MDA-MB-231 cells were exposed to 0, 10 or 20 μM GLA for 2 days. RT-PCR analyses and (c) relative mRNA levels of E-cadherin and vimentin were determined (mean ± SD, n = 3). MDA-MB-231 cells were exposed to 10 μM GLA for 0, 8, 16, 24 or 48 h. Western blot analyses (d) and relative protein levels (e) of E-cadherin and vimentin (mean ± SD, n = 3). BT-549 cells were exposed to 0, 1, 5, 10 or 20 μM GLA for 48 h. (f) Western blot analyses and (g) relative protein levels of E-cadherin and vimentin (mean ± SD, n = 3).