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. 2014 Feb 11;105(3):324–333. doi: 10.1111/cas.12348

Figure 3.

Figure 3

Gli1 contributes to the augmentation of malignant potential by reoxygenation. (a) Cell number in control or Gli1 siRNA-transfected-reoxygenated Ch-H-R cells was counted (n = 3). (b) Invaded cell number in control or Gli1 siRNA-transfected-reoxygenated Ch-H-R cells was investigated (n = 3). (c) Reoxygenated Ch-H-R cells were transfected with control, MMP2 or MMP9 siRNA. Then the invaded cell number in these cells was counted (n = 3). (d) MMP2 and MMP9 mRNA expression in Gli1 siRNA transfected-reoxygenated Ch-H-R cells were evaluated by real-time RT-PCR (n = 3). The graph shows mean ± SD. *P < 0.05.