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. 2014 Feb 11;105(3):324–333. doi: 10.1111/cas.12348

Figure 5.

Figure 5

Reoxygenation enhances tumorigenicity by Gli1 in vivo. (a) Colony number in wild type cells and reoxygenated Ch-H-R cells was evaluated (n = 3). (b) Wild-type cells cultured under normoxia and reoxygenated Ch-H-R cells derived from AsPC-1 were implanted into flank regions of BALB/c nude mice. Tumor volume was evaluated at the indicated day (left panel) (n = 6). Representative images of tumor-bearing mice (right panels). (c) GLI1 expression in tumors from each mouse was determined by immunofluorescence staining (upper panels) (×630). The graph shows relative ratio of GLI1 positive cells in tumors (lower panel). (d) Colony number in Gli1 siRNA transfected-reoxygenated Ch-H-R cells was estimated (n = 3). (e) Control or Gli1 siRNA-transfected-reoxygenated Ch-H-R cells derived from AsPC-1 were implanted into flank regions of BALB/c nude mice. Tumor volume was evaluated on the indicated day (n = 6). The graph shows mean ± SD. *P < 0.05.