Table 2.
Detection and comparison of STAT3 gene mutations using direct sequencing, AS-PCR and AS-qPCR methods
| UPN | Mutation detection method |
||||
|---|---|---|---|---|---|
| Direct sequencing | AS-PCR Y640F | AS-PCR D661Y | AS-qPCR Y640F (%) | AS-qPCR D661Y (%) | |
| J1 | − | + | − | 3.42 | ND |
| J2 | − | + | − | 2.16 | ND |
| J3 | − | + | + | 6.81 | 1.11 |
| J8 | − | − | + | ND | 0.96 |
| J9 | Y640F | + | − | 21.3 | ND |
| J12 | − | − | + | NA | NA |
| J13 | − | − | + | ND | 0.57 |
| J19 | D661Y | + | + | 6.35 | 25.90 |
| J22 | D661Y | − | + | ND | 34.60 |
| J32 | − | − | + | ND | 0.03 |
| J34 | − | − | + | ND | 1.97 |
| J35 | − | − | + | ND | 0.10 |
| J38 | D661Y | − | + | ND | 34.50 |
| H1 | − | + | − | 1.24 | ND |
| H3 | − | − | + | ND | 12.10 |
| H4 | − | − | + | ND | 0.62 |
| H5 | − | − | + | ND | 7.27 |
| H10 | − | − | + | ND | 1.79 |
| H16 | − | − | + | ND | 0.15 |
| H17 | − | + | − | 0.61 | ND |
| H22 | − | − | + | ND | 0.13 |
| H26 | D661Y | − | + | ND | 17.80 |
| H30 | − | − | + | ND | 0.18 |
| NC | − | − | − | ND | ND |
AS-PCR, allele-specific PCR; AS-qPCR, allele-specific quantitative PCR; NA, not available; NC, normal control; ND, not detected; PCR, polymerase chain reaction; STAT3, signal transducer and activator of transcription 3. −, negative for the mutation(s); +, positive for the mutations.