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6-carboxyfluorescein (FAM)-labeled siRNA (FAM-siRNA)/CH-CA-Spe nanogel complex was endocytosed by tumor cells. Renca cells were transfected with 6-carboxyfluorescein (FAM)-labeled siRNA by means of CH-CA-Spe nanogel or cationic liposome. Three (a) and seven (b) hours later, lysosomes were stained with LysoTracker Blue DND-22 (Life Technologies) and visualized by confocal laser scanning microscopy. Scale bars represent 10 μm.
