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. 2015 Jan 7;17(1):2. doi: 10.1186/s12575-014-0012-4

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© Abdelaal et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Comparison of fresh lymphoid tissue sections cut with a Compresstome or Vibratome. Fresh unfixed lymph node (A-B) and spleen (C-D) tissue sections were generated with either a Compresstome (left panels) or Vibratome (right panels) and processed for in situ tetramer staining combined with immunohistochemistry. Tissue sections were stained with Mamu-A1/Gag (CM9) MHC-tetramers to label SIV-specific CD8 T cells (red to pinkish color), anti-CD20 antibodies to detect B cells (green), and anti-CD3 antibodies to detect T cells (Blue). Confocal mages were collected using a 20 X objective and 3 um z-steps. The upper left panel of each image shows a montage of several projected confocal z-series fields. Scale bars = 100 microns. We used the curves tool in Photoshop to increase the contrast of each image, and each image was adjusted similarly.