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. Author manuscript; available in PMC: 2015 Feb 5.
Published in final edited form as: J Control Release. 2010 Jul 7;148(2):219–225. doi: 10.1016/j.jconrel.2010.06.017

Fig. 1.

Fig. 1

The presence of serum does not negatively impact LPDII-mediated transfection in a macrophage-like cell line. Plasmid DNA was condensed with protamine at a 1.2:1 weight ratio and incubated with P388D1 cells either alone (P-D), or after further complexation with either LLO-containing liposomes (LLO-LPDII) or Lipofectamine. As a negative control, heat-inactivated LPDII (HI-LPDII) were formulated by heating LLO-containing liposomes at 75 °C for 10 min. Plasmid DNA in various forms was incubated with cells at 1 μg per well under all conditions. The results are expressed as relative light units (RLU) of luciferase reporter gene expression per milligram of total cellular protein (n = 3).