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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Cell. 2014 Jan 30;156(4):649–662. doi: 10.1016/j.cell.2014.01.020

Figure 4. Accelerating HSPC cycling increases reprogramming efficiency and induces the emergence of privileged cells.

Figure 4

(A) The cell cycle speed of LKS and GMP cells were measured, either fresh (black bars) or after 5 days of culture (red bars). The percentage of cells with indicated cell cycle speed is plotted (details in Extended Experimental Procedures and Figure S4). Note that fresh GMPs and cultured LKS cells contain ultrafast cycling cells (red boxes), which were low/absent in fresh LKS cells and cultured GMPs. (B) The reprogramming efficiency of fresh or cultured LKS and GMP cells were compared in single cell reprogramming assays. The number of wells that contained Oct4:GFP+ colonies and the total number of plated wells are indicated, separated by a slash. (C) Singly sorted LKS cells were cultured for 5 days and its somatic progeny transferred into reprogramming conditions for another 5 days (scheme shown in Figure S5B). Shown is one representative well dominated by Oct4:GFP+ colonies and lacked hematopoietic-like cells. ~15% of the wells containing Oct4:GFP+ colonies (or 3.6% of total wells) displayed this privileged reprogramming behavior. Images were captured with 10x magnification. See also Figures S4, S5 and Tables S3, S4.