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. Author manuscript; available in PMC: 2015 Feb 13.
Published in final edited form as: Cell. 2014 Jan 30;156(4):649–662. doi: 10.1016/j.cell.2014.01.020

Figure 6. The ultrafast cycling population from MEFs is increased by p53 knockdown and account for most reprogramming activity.

Figure 6

MEFs were transduced with either control (shCtrl) or shRNAs targeting p53 (shp53), along with the Dox-inducible reprogramming factors. (A) p53-knockdown increased reprogramming in un-fractionated MEFs. (B) Western blot analysis confirmed p53 protein down-regulation by p53-targeting shRNAs. (C–D) Factor-transduced MEFs on 4 days of Dox treatment were labeled with CFSE (top) and allowed to dilute the dye for another 48 hours. The cultures were then trypsinized and CFSE intensity analyzed by FACS. (C) Representative FACS plots are shown for CSFE levels right after labeling, or following 48 hours of dye-dilution. Gating for fast and slow cells are shown. (D) Quantification of fast cycling cells (n=3 per condition). (E) Reprogramming efficiency of the sorted fast and slow cycling MEFs as shown in (C) (n=3 per condition). Error bars indicate standard deviation.