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. 2015 Feb 5;6:46. doi: 10.3389/fpls.2015.00046

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Copyright © 2015 Hussain, Shah, Rahman, Irshad and Iqbal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tryptophan dependent production and release of IAA by wild (Os-1), ipdC knockout mutant (ΔipdC) and complementation strain (ΔipdCpipdC) of Nostoc over time. IAA was determined in the culture filtrate and Nostoc filaments by UPLC-ESI-MS/MS after incubation of time indicated. Graph represents data from three replicates in two independent experiments (data are mean ± SD; n = 6). Asterisk indicate a statistically significant difference (*p < 0.001; 3-way ANOVA) relative to the respective means of wild type and complementation strains.