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. 2015 Feb 5;6:46. doi: 10.3389/fpls.2015.00046

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Copyright © 2015 Hussain, Shah, Rahman, Irshad and Iqbal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of exogenous IAA on the in vitro growth of free grown and root colonized Nostoc. To reconstitute the concentration of IAA in mutant strain (ΔipdC), IAA was supplemented to the culture media used to grow mutant Nostoc alone or in association with rice root in concentration exactly equal to (ΔipdC+IAA) or double than (ΔipdC +2IAA) the difference in its concentration produced by wild and mutant strains. Growth and colonization of Nostoc was determined by two methods, (A) quantification of chlorophyll-a and (B) counting Nostoc on agar plate (cfu). Graph represents data from nine replicates in two independent experiments (data are mean ± SD; n = 18). Similar bars labeled with different letters shows significant difference (p < 0.05; Duncan test).