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. 2015 Feb 5;9:29. doi: 10.3389/fncel.2015.00029

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Copyright © 2015 Paul, Pauli, Ehmann, Hallermann, Sauer, Kittel and Heckmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Type Ib bouton morphometry in syt^KD. (A) syt^KD NMJ stained with α-HRP against neuronal membranes (magenta) and mAb Brp^Nc82 (green). Higher magnification of type Ib ending (white box) showing the three terminal boutons. Scale bars = 20 μm, 2 μm (inset). (B) Summary bar graph for the number of Brp puncta per terminal three type Ib boutons in syt^KD and (C) bouton area, length, and width.