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. 2015 Jan 23;14:11. doi: 10.1186/s12934-015-0194-8

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© Wang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Confirmation of in-frame deletion of prodigiosin biosynthesis genes in DSM 6842. (A) PCR detection of pigM-K single crossover mutants using two primer pairs. M, DNA Marker III. 1–6, six independent colonies that can grow on the selective plate after conjugation. WT, DSM 6842 wild-type strain. NC, negative control ddH₂O. (B) PCR confirmation of the mutants that underwent a second homologous recombination using the pigM-wS and pigM-wA primers. 1–3, three independent colonies after the second homologous recombination. WT, wild-type strain DSM 6842. NC, negative control ddH₂O. (C) The prodigiosin production of three ΔpigM-K mutants and the wild-type strain DSM 6842.