Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jan 23;14:11. doi: 10.1186/s12934-015-0194-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Wang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Confirmation of in-frame deletion of hmgA in SCSIO 04301. (A) PCR detection of hmgA single crossover mutants using two primer pairs. M, DNA Marker III. PCR templates used are: 1, one colony that can grow on the selective plate after mating. WT indicates SCSIO 04301 wild-type strain. NC indicates negative control ddH₂O. (B) PCR confirmation of the mutants that underwent a second homologous recombination using the hmgA-wS and hmgA-wA primers. 1, one colony after the second homologous recombination. WT indicates SCSIO 04301 wild type. NC indicates negative control ddH₂O. (C) The pigment production of wild-type SCSIO 04301, ΔhmgA mutant, ΔhmgA/pWD2-oriT and ΔhmgA/pWD2-oriT-hmgA.