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. 2015 Jan 23;14:11. doi: 10.1186/s12934-015-0194-8

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© Wang et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Confirmation of in-frame deletion of flagellar motor protein genes in SCSIO 11900. (A) PCR detection of fliFG single crossover mutants using two primer pairs. M, DNA Marker III. PCR templates used are: 1 and 2, two independent colonies that can grow on the selective plate after mating. WT indicates wild-type SCSIO 11900. NC indicates negative control ddH₂O. (B) PCR confirmation of the mutants that underwent a second homologous recombination using the fliFG-wS and fliFG-wA primers. 1, one colony after the second homologous recombination. WT indicates wild-type SCSIO 11900. NC indicates negative control ddH₂O. (C) Swimming motility of wild-type SCSIO 11900 and ΔfliFG.