Table 1.
Bacterial strains and plasmids used in this study
| Strains/plasmids | Description a | Reference |
|---|---|---|
| Pseudoalteromonas strains | ||
| SM9913 | P. sp. SM9913, deep-sea sediment at a water depth of 1855 meters near the Okinawa Trough, 20°C | [8] |
| A37-1-2 | P. arctica A37-1-2, Arctic sea ice strain, 20°C | [7] |
| DSM 16099 | P. spiralis DSM 16099, deep ocean hydrothermal vents of the Juan de Fuca Ridge, 30°C | [35] |
| DSM 16098 | P. telluritireducens DSM 16098, deep ocean hydrothermal vents of the Juan de Fuca Ridge, 30°C | [35] |
| DSM 6842 | P. rubra DSM 6842, Mediterranean coastal waters off Nice, 25°C | [26,36] |
| SM20429 | P. sp. SM20429, plasmid curing mutant of P. sp. BSi20429, Arctic sea ice strain, 20°C | [9] |
| TAC125 | P. haloplanktis TAC125, Antarctic surface seawater, 20°C | [37] |
| SCSIO 04301 | P. lipolytica SCSIO 04301, sediment at 63 m deep in the South China Sea (18°0=N, 109°42=E), 25°C | [28] |
| SCSIO 11900 | P. sp. SCSIO 11900, surface mucus layer of the coral at 4 m deep in the South China Sea (18°13=N, 109°28=E) , 25°C | [28] |
| ΔbsmA | bsmA gene deletion mutant of P.sp. SM9913 | This study |
| ΔpigM-K | Deletion mutant of the DNA region containing pigM-K genes related to prodigiosin biosynthesis in P. rubra DSM 6842 | This study |
| ΔhmgA | hmgA gene deletion mutant of P. lipolytica SCSIO 04301 | This study |
| ΔfliFG | Deletion mutant of the DNA region containing fliFG genes encoding flagellar motor proteins in P. sp. SCSIO 11900 | This study |
| Escherichia. coli strain | ||
| WM3064 | RP4 (tra) in chromosome, DAP-, 37°C | [23] |
| Plasmids | ||
| pWD2 | E. coli and Pseudoalteromonas shuttle vector, Ampr, Cmr | [9] |
| pHT304 | E. coli and Bacillus thuringiensis shuttle vector, Ampr, Eryr thuringiensis shuttle vector, Ampr, Eryr | [30] |
| pBBR1MCS-2 | Broad-host-range cloning vector, Kanr | [22] |
| pWD2-oriT | pWD2 containing 1.5 kb BamHI fragment with mobilization region from pBBR1MCS-2, Ampr, Cmr | This study |
| pWD2Ery-oriT | pWD2-oriT containing a 900bp erythromycin resistant gene replaced the chloramphenicol resistant gene, Ampr, Eryr | This study |
| pK18mobsacB | Widely used gene knockout vector, Kanr | [24] |
| pK18mobsacB-Cm | pK18mobsacB containing the chloramphenicol resistant gene from pWD2, Kanr, Cmr | This study |
| pK18mobsacB-Ery | pK18mobsacB containing the erythromycin resistant gene from pHT304, Kanr, Eryr | This study |
| pK18Cm-bsmA | pK18mobsacB-Cm containing the homologous arms of bsmA gene of SM9913, Kanr, Cmr | This study |
| pK18Ery-pigM-K | pK18mobsacB-Ery containing the homologous arms of the pigM-pigK DNA region of DSM 6842, Kanr, Eryr | This study |
| pK18Ery-hmgA | pK18mobsacB-Ery containing the homologous arms of hmgA gene of SCSIO 04301, Kanr, Eryr | This study |
| pK18Ery-fliFG | pK18mobsacB-Ery containing the homologous arms of the fliF-fliG DNA region of SCSIO 11900, Kanr, Eryr | This study |
aAmpr, ampicillin resistance; Eryr, erythromycin resistance; Kanr, kanamycin resistance; Cmr, chloramphenicol resistance.
Temperature indicates the optimal growth temperature of each strain.