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. 2014 Dec 14;11:66. doi: 10.1186/s12989-014-0066-0

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© Sanpui et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Changes in expression of IL-8, RANTES and IFIT proteins. SAEC were singly exposed to 50 μg/ml SWCNTs, 5.0 MOI IAV or pre-treated with 50 μg/ml SWCNTs for 24 h followed by exposure to 5.0 MOI IAV for 24 h. The levels of (A) IL-8 and (B) RANTES were quantified by ELISA in cell media and data presented as the mean pg/ml ± standard error for three separate experiments. Significant differences in expression levels were determined by ANOVA; *compared to control for each treatment; + significant differences between treatments (P < 0.05) (C). The levels of IFIT3 and IFIT2 were assessed by Western Blot analysis. The expected molecular weight of IFIT3 and IFIT2 is 58 kDa and 55 kDa, respectively. These corresponding areas are marked with an arrow for each blot. The unmarked bands are non-specific targets. The data show the most intense bands for IFIT3 in cells treated with IAV whereas no bands were detectable for IFIT2.