Adenovirus-mediated transfection with Akt knockout can modulate alcohol effects on the Akt/PI3k pathway. (A–D) SOD-3, NFkB, ERK1, and p38 relative gene expression with acute low, moderate, and high levels of alcohol treatment along with adenovirus Ad.Akt (K179M, Akt knockout). Fold change in expression shown as compared to control samples (*) with p-values < 0.05. All Ad:EGFP control samples were equivalent to control nontransfected samples.