Table 2.

Required video duration and number of replicates for identifying a significant effect

Plant-aphid system	Duration	Replicates	Detection rate
Arabidopsis - M. persicae	8 h	20	> 80%
	6 h	25	> 80%
Lettuce – N. ribisnigri	8 h	20	> 80%
	4 h	15	> 80%

Student’s t-tests have been applied to subsamples of two simulated data sets with a similar mean and standard deviation as two response variables from the video tracking assays. The percentage of tests with a significant outcome in at least one of the two variables is represented as the detection rate (Bonferroni correction: P < 0.025).