Figure 5.

Interaction of gp5_βf proteins with full-length, wild-type IAPP. (A) Data for a representative fiber formation reaction, monitored using fluorescence of 200 nM ThT, are shown with addition of 1 nM (blue), 10 nM (green), or 100 nM (orange) gp5_NGIS to 50 μM IAPP. Data for the IAPP-only reaction are colored red. (B) Statistics of reaction midpoints, t₅₀, relative to IAPP-only t₅₀ (red) from fits to repeats of kinetic measurements such as in panel A, but using the indicated gp5_βf variant. (C) Toxicity of IAPP toward INS-1 cells at 13 μM IAPP alone and with the indicated gp5_βf molecule at 0.5 μM (white bars). The toxicities of the indicated gp5_βf proteins alone at 0.5 μM are shown as gray bars. ND in panel B indicates no detected change after observation for 16 h.