Fig.2.

ResMap analysis of His-β-L HBcAg VLP reconstructions. ResMap was used to evaluate the local resolution of both T = 3 (A) and T = 4 (B) reconstructions. Isosurfaced representations of the reconstructions were coloured accordingly revealing that the HBcAg component was solved at higher resolution than the N-terminal extension. In the case of the T = 3 particle the core density achieved resolution approaching 5 Å, ranging to 8 Å in places, while the inserted polypeptide was seen to be poorer than 19 Å resolution. The T = 4 reconstruction was calculated from fewer particles and achieved lower resolution ranging between 8 and 10 Å in the HBcAg region and 11–>19 Å in the N-terminal extension. Interestingly both ResMap analyses gave resolution assessments that included small patches of poor-resolution throughout the structure, in particular within the dimeric spike of HBcAg, these are apparent in clipped isosurface representations (C and D).