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. 2015 Feb;189(2):114–122. doi: 10.1016/j.jsb.2014.11.010

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© 2014 The Author

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig.2 — Improved GUI and workflow. (A) Screenshot of the new GUI in RELION-1.3. (B) Proposed workflow for semi-automated particle selection in RELION-1.3. After CTFs have been estimated for all micrographs, the particle selection procedure consists of five steps (numbered 1–5), as explained in more detail in Section 4.