Fig.3.

Particle selection for the KLH data. (A) The ten reference-free class averages (ordered from larger to smaller classes) that were calculated from the manually selected particles. The two classes indicated with an asterisk were selected as templates for the auto-picking. (B) Curves of precision, recall and false discovery rate against the pick threshold. A picking threshold of 0.3 was chosen. (C) The 15 particles with the highest average Z-scores after sorting. (D) The 15 largest classes (ordered from larger to smaller) after 2D class averaging of the auto-picked particles. Particles assigned to the classes indicated with an asterisk were selected for subsequent 3D refinement. (E) 3D map after refinement of the semi-automatically selected particles from the combined near-to-focus (NTF) and far-from-focus (FFF) KLH data sets.