Fig.4.

Particle selection for the $\beta$-galactosidase data. (A) The 25 class averages that were calculated from the manually selected particles (ordered from larger to smaller class). The 10 class averages indicated with an asterisk were selected as templates for the auto-picking. (B) Curves of precision, recall and false discovery rate against the pick threshold. A picking threshold of 0.4 was chosen. (C) Map obtained after 3D refinement with the manually picked particles. (D) Map obtained after 3D refinement with the semi-automatically selected particles.