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Multiplexing comparison. DiLeu-labeled yeast lysate digest samples were prepared as 4-, 8-, and 12-plex mixtures in 10:1 ratios between neighboring channels and analyzed by LC–MS2 at 60k resolving power. Measured quantitative ratios of identified proteins (box and whiskers) are shown. Box plots demarcate the median (line), the 25th and 75th percentile (box), and the 5th and 95th percentile (whiskers).
