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POC2 and VPS4 are localized at the TZ. Isolated flagella/basal body complexes (upper panels) stained for acetylated α-tubulin (green) show a pair of POC2 (left, red) and VPS4 (right, red) puncta at their base. Immunostaining of TZs in isolated cell walls (lower panels) confirms the presence of POC2 and VPS4 in the TZ.
