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. Author manuscript; available in PMC: 2016 Feb 21.
Published in final edited form as: Org Biomol Chem. 2015 Feb 21;13(7):2078–2086. doi: 10.1039/c4ob02309a

Figure 4.

Figure 4

Trafficking assays of the β2-adrenergic receptor (β2AR). A. Sequential treatment of HEK-293 cells stably expressing a HA-FAP-β2AR construct with isoproterenol (iso) and alprenolol (alp) or alprenolol and latrunculin B (alp + lat), allows separation of receptor endocytosis and recycling. B. Antibody labeling of the surface exposed HA epitope reveals desensitization and recycling levels, nearly identical to (C) those measured by fluorogenic dye addition in a simple “add and read” assay format with improved precision in the simplified assay. The error bars represent SEM of 3 independent replicate experiments on different days.