Fig. 7.

Zinc and copper enhance the cytotoxicity of disulfiram. (A) Cells were treated with a serial dilution of zinc or copper for 72 h in the presence of complete growth media before cell viability analysis was performed. (B) Cells were treated with disulfiram ±20 μM zinc or copper in complete media for 72 h prior to performing viability analysis. (C and D) Viability of MCF-7 cells treated with disulfiram ± zinc or copper in supplemented in complete media was analyzed after 72 h. Error bars show standard error. p-values for data in Fig. 7B are provided in Table 1.