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. 2015 Feb 1;4:48. doi: 10.1186/s40064-015-0826-9

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© Sekine et al.; licensee Springer. 2015

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Dose-dependent inhibition of KYNA production in tissue slices from the cerebral cortex by (a) leucine (Leu), (b) isoleucine (Ile), (c) phenylalanine (Phe), (d) tyrosine (Tyr), (e) methionine (Met), (f) cysteine (Cys), (g) aspartate (Asp), (h) glutamine (Gln), (i) alanine (Ala), and (j) glutamate (Glu). Experiments were performed as described in the text using 2 μmol/L KYN. KYNA was measured in incubation medium. Values are expressed as mean ± SE (n = 4–6). Sigmoid curves were generated by nonlinear regression analysis using Graph Pad Prism 5.0.